Molecular epidemiology of infectious bursal disease virus in Zambia.
نویسندگان
چکیده
Nucleotide sequences of the VP2 hypervariable region (VP2-HVR) of 10 infectious bursal disease viruses detected in indigenous and exotic chickens in Zambia from 2004 to 2005 were determined. Phylogenetic analysis showed that the viruses diverged into two genotypes and belonged to the African very virulent types (VV1 and VV2). In the phylogenetic tree, strains in one genotype clustered in a distinct group and were closely related to some strains isolated in western Africa (VV1), with nucleotide similarities of 95.7%- 96.5%. Strains in the other genotype were clustered within the eastern African VV type (VV2), with nucleotide similarities of 97.3%- 98.5%. Both genotypes were distributed in the southern parts of Zambia and had a unique conserved amino acid substitution at 300 (E→A) in addition to the putative virulence marker at positions 222(A), 242(I), 256(I), 294(I) and 299(S). These findings represent the first documentation of the existence of the African VV-IBDV variants in both indigenous and exotic chickens in Zambia.
منابع مشابه
Pathogenicity Study and Restriction Enzyme Profile of a Recently Isolated Infectious Bursal Disease Virus in Iran
The pathogenicity of a recent isolate of Infectious bursal disease virus (IBDV), IR499, isolated from a nonvaccinated flock with 17.5 % mortality rate in susceptible SPF chickens, chickens embryos and broilers was discussed. The molecular characterization of the virus based on the restriction fragment length polymorphism (RFLP) pattern was also investigated. The mortality rates were 85% and 22%...
متن کاملIsolation, Characterization and Standardization of New Infectious Bursal Disease Virus for Development of a Live Vaccine
Background and Aims: Infectious bursal disease (IBD) is an acute contagious viral disease of birds worldwide. The causative virus induces a persistent immune suppression following destroy B lymphocytes precursors in bursal lymphoid follicles. Vaccination is the main strategy for prevention of the disease in commercial poultry industry. Materials and Methods: To produce a live vaccine against th...
متن کاملEffect of experimental infectious bursal disease virus on clinical signs and pathogenesis of avian influenza virus H9N2 in turkey by real time PCR
Infectious bursal disease virus (IBDV) in turkeys may result in immunosuppression, and inability of turkeys to resist nonpathogenic or less pathogenic organisms. A total number of 120 day-old commercial male turkeys were purchased and blood samples were collected from 20 day-old turkeys, remaining 100 were divided into four equal groups and kept in separated rooms. Groups 1 and 2 were infected ...
متن کاملCrude extract of bacterially expressed, infectious bursal disease virus VPX/VP2 proteins induces protective immune response to the virus in chickens
Infectious bursal disease virus (IBDV) infects young chickens and causes serious lose to the poultry industry, worldwide. Previous attempts using purified bacterially expressed IBDV VP2 failed to elicit a protective immune response to the virus. This study was designed to investigate if the initial expressed protein contained neutralizing epitopes but became nonfunctional during purification st...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of the South African Veterinary Association
دوره 84 1 شماره
صفحات -
تاریخ انتشار 2013